QotNews Hacker News, Reddit, Lobsters, and Tildes articles rendered in reader mode.
CBD good for HIV-infected brain cells: A new study shows CBD works at the molecular level to reduce HIV-associated neuroinflammation, keeping infected cells under control so they don’t activate and spread the virus.
>HC69.5 (HIV/GFP) cells were donated by Dr. Karn’s lab. Dr. Karn’s lab team infected the cells with HIV reporter viruses. Only latently infected clones reactive to HIV in response to inflammatory signals were selected. HC69.5 (HIV/GFP) cells were rigorously characterized, and therefore provide a vital resource for the study of HIV infection in microglial cells. Cells were grown in DMEM medium supplemented with 1% FBS in a 37 °C incubator with 5% CO2. We selected the addition of poly IC (100 µg/ml) as an activator following previous work published by Alvarez- Carbonell12,29 C20, a Human microglia cell line was donated by Dr Karn’s lab and used as control12
>XTT cell viability assay
>The XTT cell viability assay was performed to confirm that the CBD and Δ (9)-THC concentrations used were not toxic as described. Cell proliferation and viability were evaluated by the XTT assay (Cat 11465015001; Sigma Aldrich; Roche) after 24 h incubation with Δ (9)-THC and CBD (0.01 μM; 0.1 μM; 1 μM; 5 μM, 10 μM) compounds. The XTT assay was measured at 475 nm and 660 nm, using a Biotek Synergy HT multimode microplate reader instrument after the addition of XTT reagent. Results are expressed as a percentage of cell viability compared to control cells. Statistical significances were calculated by ANOVA and Dunn’s Multiple Comparison Test as a post hoc test.
Nearly impossible to replicate in vivo. I’d be interested to see if HIV patients who smoked weed or took CBD had reduced viral loads as compared to those who don’t (double blind if possible).
This reminds me of a very active investigation and grass-roots experimenting with nicotine patches and chronic fatigue syndrome.
civver3 | 2 years ago
>Cell culture
>HC69.5 (HIV/GFP) cells were donated by Dr. Karn’s lab. Dr. Karn’s lab team infected the cells with HIV reporter viruses. Only latently infected clones reactive to HIV in response to inflammatory signals were selected. HC69.5 (HIV/GFP) cells were rigorously characterized, and therefore provide a vital resource for the study of HIV infection in microglial cells. Cells were grown in DMEM medium supplemented with 1% FBS in a 37 °C incubator with 5% CO2. We selected the addition of poly IC (100 µg/ml) as an activator following previous work published by Alvarez- Carbonell12,29 C20, a Human microglia cell line was donated by Dr Karn’s lab and used as control12
>XTT cell viability assay
>The XTT cell viability assay was performed to confirm that the CBD and Δ (9)-THC concentrations used were not toxic as described. Cell proliferation and viability were evaluated by the XTT assay (Cat 11465015001; Sigma Aldrich; Roche) after 24 h incubation with Δ (9)-THC and CBD (0.01 μM; 0.1 μM; 1 μM; 5 μM, 10 μM) compounds. The XTT assay was measured at 475 nm and 660 nm, using a Biotek Synergy HT multimode microplate reader instrument after the addition of XTT reagent. Results are expressed as a percentage of cell viability compared to control cells. Statistical significances were calculated by ANOVA and Dunn’s Multiple Comparison Test as a post hoc test.
Okay, how does it work in vivo?
SpicySweett | 2 years ago
Nearly impossible to replicate in vivo. I’d be interested to see if HIV patients who smoked weed or took CBD had reduced viral loads as compared to those who don’t (double blind if possible).
This reminds me of a very active investigation and grass-roots experimenting with nicotine patches and chronic fatigue syndrome.